FIG. 2.
Phylogenetic analysis of RDH genes from Dehalococcoides sp. strain CBDB1 (rdhA1-14CBDB1), Dehalococcoides sp. strain FL2 (rdhA1-11FL2), Dehalococcoides sp. strain BAV1 (rdhA1-7BAV1), and D. ethenogenes strain 195 (rdhA1-17DE) (48). The neighbor-joining tree shown was generated from amino acid sequences of nearly complete orfA genes (see Fig. 1 and text for details). Branching points supported by 85 to 100% of 1,000 bootstrap sampling events are indicated by solid circles. Open circles indicate 50 to 84% support by bootstrap sampling. Branching points supported by the maximum parsimony treeing method are marked with (P). Additional clones from strain FL2 (rdhA*FL2, sequences not determined) are indicated together with the rdhACBDB1 genes that have identical restriction patterns. Dotted double, triple, and quadruple lines indicate subclusters of highly similar genes from two, three, or four different Dehalococcoides strains, respectively (see the text for details). tceA, trichloroethene reductive dehalogenase, pceA, tetrachloroethene reductive dehalogenase, cprA,chlorophenol reductive dehalogenase. The tceA cluster comprises the tceA genes of D. ethenogenes strain 195 (GenBank accession number AAF73916), strain FL2 (accession number AY165309), and three chloroethene-dechlorinating enrichment cultures (accession numbers AAN85590, AAN85592, and AAN85594). The scale bar represents 20% sequence divergence.