FIG. 5.
Restoring effect of Mg2+ on the growth of S. yanoikuyae EC-S001 in PD medium. (a) Amounts of each ingredient added to plain PD medium were equal to concentrations in the original Hoagland's no. 2 mineral solution. Among the ingredients of Hoagland's no. 2 mineral medium, only 0.98 mM MgSO4 · 7H2O allowed cell growth of EC-S001. (b) To examine the threshold level of Mg2+ for EC-S001 cell growth, a series of concentrations of MgSO4 · 7H2O was added to PD medium. Values are averages from duplicate experiments after 48 h of incubation.