Table 2.
K m and V max values of GDH towards different substrates
| Substrate | K m (mol/L) | V max [U (mg protein)−1] | Substrate | K m (μmol/L) | V max [U (mg protein)−1] |
|---|---|---|---|---|---|
| Methanol | – | – | Ethanol | – | – |
| 1-propanol | – | – | NADP+ | 0.07 | 0.083 |
| n-butanol | – | – | NADPH | 0.13 | 0.182 |
| Isobutanol | – | – | α-ketoglutarate | 4.01 | 0.14 |
| hexanol | 20.37 | 16.13 | Glutamate | 41.74 | 0.87 |
| Isoamyl alcohol | 19.37 | 5.59 | NH4 + | 5.35 | 0.003 |
The K m and V max values for each substrate were determined by measuring the initial reaction rates at various non-saturating concentrations of the substrate in the presence of a fixed volume of enzyme
–Indicates not detected