Fig. 1.

Construction of the transformation vector pAN7-1-Chi67-1. a gpdA and trpC fragments, which were cloned from plasmid pAN7-1, and the Chi67-1 fragment cloned from the C. rosea strain 67-1 genome were ligated to the linearized pAN7-1, which was digested with HindIII to construct the transformation vector pAN7-1-Chi67-1. b PCR amplification of inserted fragments. M DNA marker; 1 gpdA; 2 Chi67-1; 3 trpC. c verification of transformation vector. 1 pAN7-1; 2, 3: constructed vector pAN7-1-Chi67-1