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. 2017 Jan 3;7:1. doi: 10.1186/s13568-016-0313-x

Table 1.

Primers used in this study

Primer	Sequence (5′–3′)	Purpose
ChiF	GTTGTGGTTTGCCTGGTG	Real-time PCR
ChiR	CCGATACTCTGCTGCTCAT	Real-time PCR
hphF	ATGCCTGAACTCACCGCGACGTCTG	Hygromycin amplification
hphR	CTATTCCTTTGCCCTCGGACGAGTG	Hygromycin amplification
CF	TTCAGAGTAGGCTTTTGGATTGGT	Chi67-1 amplification
CR	ACCCCATATTTGCTCATAATCACA	Chi67-1 amplification
qdzF	ACTCGACCTGCAGGCATGCAAGCTTGAATTCCCTTGTATCTCTACACA	gpdA amplification
qdzR	ACCAATCCAAAAGCCTACTCTGAAGGGAAAAGAAAGAGAAAAGAAAAG	gpdA amplification
zzzF	TGTGATTATGAGCAAATATGGGGTGGATCCACTTAACGTTACTGAAA	trpC amplification
zzzR	GTAAACGACGGCCAGTGCCAAGCTTTCGAGTGGAGATGTGGAGTGG	trpC amplification