Figure 1.

Dark- and Light-Grown spa2 Mutant Seedlings Are Indistinguishable from the Wild Type.

(A) Molecular analysis of the spa2-1 mutant. The top panel shows the SPA2 transcript splicing model and the location of the T-DNA insertion site in spa2-1. The bottom left panel shows the RNA gel blot analysis of RNA isolated from Rc-grown wild-type and spa2-1 mutant seedlings. The blot was hybridized with a SPA2-specific probe. The bottom right panel shows an RT-PCR analysis of RNA isolated from wild-type and spa2-1 mutant seedlings. RNA was reverse transcribed and subsequently amplified by PCR using primers flanking the T-DNA insertion site in spa2-1 or, as a control, SPA3-specific primers.

(B) to (D) Hypocotyl length of wild-type and spa2-1 mutant seedlings grown in Rc (B), FRc (C), or Bc (D) of various fluence rates. Error bars denote one standard error of the mean.