Molecular Analysis of DNA from T1 Progeny of the TAC26 Transgenic Petunia Lines.
(A) to (C) Genomic DNA gel blot analysis of the T1 progeny (b1 to b3), T0 transformant b, and the untransformed control. Leaf DNA digested by HindIII was blotted and probed with the Hyg fragment (A), AhSLF-S2 (B), and AhS2-RNase (C), respectively.
(D) to (F) DNA gel blot analysis of the T1 progeny (l1 to 5, 7, and 8), T0 transformant, and the untransformed control. Leaf DNA digested by HindIII was blotted and probed with the Hyg fragment (D), AhSLF-S2 (E), and AhS2-RNase (F), respectively. Sizes of the hybridizing fragments are indicated in kilobase pairs.