Figure 5.

Molecular Analysis of AhSLF-S₂ Transgenic Petunia Plants.

(A) A schematic diagram of pBIAhSLF-S₂. RB and LB, right and left borders of the T-DNA; Km^r, kanamycin resistance (neomycin phosphortransferase) gene. Several restriction sites also are indicated.

(B) and (C) DNA gel blot analysis of the transgenic lines WQ-1, WQ-2, WQ-3, WQ-4, and WQ-5 and the untransformed control (WT). Leaf genomic DNA was digested with HindIII (B) or EcoRI (C) was blotted and probed with AhSLF-S₂. Leaf DNA of an S₂S₄ Antirrhinum line (Ant) was as a positive control.

(D) Leaf DNA digested by EcoRI from the transgenic lines was blotted and probed with PhS₃-RNase.