Figure 8.
Amino Acid Sequence Alignment of Predicted SLF Polypeptides from Antirrhinum and Petunia and Expression Analysis of Endogenous SLF Genes in Transgenic Petunia Plants.
(A) Alignment of the predicted polypeptide sequences from the SLF family. Antirrhinum, AhSLF-S2 (CAC33010); P. inflata, PiA134-S1 (AAR15914), PiA134-S2 (AAR15915), PiA134-S3 (AAR15916), PiSLF-S1 (AY500390), PiSLF-S2 (AY500391), and PiSLF-S3 (AY500392); P. hybrida, PhSLF-S3A (AY639403) and PhSLF-S3B (AY639402). PhC1 and PhC2 indicate the conserved regions that were used to design degenerate primers for cloning PhSLF-S3A and PhSLF-S3B. The F-box domain is also indicated.
(B) and (C) RT-PCR analysis of RNA isolated from pollen of the TAC26 and AhSLF-S2 transgenic lines and wild-type plant, using specific primers of PhSLF-S3A and PhSLF-S3B with (+) or without (−) reverse transcriptase in the synthesis of cDNA. Tubulin control was the same as in Figures 4A and 6A.