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. 2017 Jan 4;7:2058. doi: 10.3389/fmicb.2016.02058

FIGURE 2.

FIGURE 2

Aeromonas dhakensis infection induces morphological and physiological changes in C. elegans. (A) Morphology of C. elegans treated with A. dhakensis AAK1 for 72 h (Top to bottom: E. coli OP50, A. dhakensis AAK1, 65°C heat-killed A. dhakensis AAK1). At 72 h, AAK1 infection resulted in total lysis of body. The corpse of AAK1 infected worm was marked by dashed line. The scale bar indicates 500 μm. (B) The body length of N2 C. elegans was significantly decreased after A. dhakensis AAK1 infection when compared with the control E. coli OP50 (P < 0.0001) by two-way ANOVA test. In contrast, 65°C heat-killed bacteria did not affect the size of the animals. The average feeding rate measured by pharyngeal movement (pumping times/min) (C) and brood size (D) of N2 worms infected with AAK1were significantly reduced, as compared with worms fed on OP50, OP50-65°C, or AAK1-65°C, ∗∗∗P < 0.0001.