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. 2017 Jan 4;7:2058. doi: 10.3389/fmicb.2016.02058

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Copyright © 2017 Chen, Chen, Ou, Lee, Wu, Ko and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Aeromonas dhakensis induces necrosis in C. elegans. (A) Relative expression levels of asp-3, asp-4, and crt-1 genes measured by quantitative real-time PCR (qPCR) in C. elegans infected by A. dhakensis AAK1 for 72 h were higher than C. elegans fed by E. coli OP50. (B) The RNAi-sensitive worms, NL2099 rrf-3 (pk1426), with knockdown of crt-1 or asp-4 by RNAi showed significantly enhanced survival after A. dhakensis infection, relative to the control animals with RNAi control (L4440). (C) The WM118 worms with depressed expression of crt-1 or asp-4 by RNAi restricted to the muscle were more resistant to A. dhakensis infection than RNAi control (L4440). ^∗∗P < 0.01; ^∗∗∗P < 0.0001.