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. 2017 Jan 4;7:2014. doi: 10.3389/fpls.2016.02014

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Copyright © 2017 Meï, Cussac, Haslam, Beaudoin, Wong, Maréchal and Rébeillé.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lipid analysis of A. thaliana cells grown under nitrogen limiting conditions for 72 h. (A) Total glycerolipid content expressed as μg of FA per mg of fresh weight. (B) Quantitative analysis of the various glycerolipids (expressed as μg of FA per mg of fresh weight). (C) Membrane lipid distribution: all glycerolipids except TAG. (D) Nile-Red staining and LSC microscopy imaging showing an accumulation of lipid droplets in MTX treated cells. The data are means ± SD of three independent biological repeats. Significant differences (P < 0.05) are shown by an asterisk and were calculated by an unpaired multiple t-test using GraphPad Prism software.