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. 2004 Sep;16(9):2394–2405. doi: 10.1105/tpc.104.023150

Table 1.

Conservation of Phosphorylation Sites in Membrane Transporters

PlantsT Family Subfamily/Name Protein Phosphorylation Site Conservation within Protein Family Conservation among Putative Orthologs (EST Database)
2.A.1 STP Monosaccharide transporter STP1 RGVDDVpSQEFDDL (+)
STP13 FVNGEKpSNGKSNG +
PHT Phosphate transporter PHT1-4 NEDNENpSNNDSRT n.f.
2.A.2 SUC Sucrose-proton symporter SUC5 AANNApTALEpTQpSpSPEDLGQ 3 (9) +
2.A.7 PUP Purine permease PUP18 EPDQILpSPRRSLE 2 (13) +
2.A.16 TDT C4-dicarboxylate/malic acid transporter (telluride resistance) At4g13800 PVFINpSGpSSRSSNS 2 (7) n.f.
At5g24030 DQLRNVpSpSENIENYLK n.f.
2.A.17 POT Proton-dependent oligopeptide transporter At3g47960 NTDVVDpSFEEEQR +
At1g69870 GSFSKSpSPpSELDV..YKRIpSpSPGSILD (+)
2.A.18 AAAT amino acid/auxin permease AtLTH4 PATPRVpSpTPEILTP +
At5g41800 PVTRLDpSDAGALF (+)
At5g01240 GRKVEDpSAAEEDI
2.A.29 Mitochondrial carrier At3g55640 KFMYMVpTGMENHK +
2.A.31 AE Anion exchangers/boron transporter At3g06450 EAPAILpSFNLKPE +
2.A.36 NHX Sodium-proton exchanger SOS1/NHX7 VNVYRRpSVSFGGI +
NHX1,2 VPFVPGpSPpTERNPPD +
2.A.40 NCS2 Nucleobase:cation symporter 2 At2g27810 GFRPKFpSGEpTTApTDpSpSSGQLSL +
2.A.49 AMT Ammonium transporter AMT1 MAGMDMpTRHGGFA 3 (6) +
2.A.55 NRAMP Metal ion (Fe/Mn) transporter NRAMP1 QLPCRVpSTSDVD +
2.A.57 ENT Equilibrative nucleoside transporter ENT6 AGIQNQpSDLpSDDDSKN 4 (7) +
2.A.72 KUP K+ uptake permease KUP2 EDDNARpSVQpSNEpSSSESR (+)
KUP11 DQKLDQpSMDEEAG

The conservation of a phosphorylatable residue (S or T) in paralogs and putative orthologs of phosphorylated transporters was analyzed. Phosphoproteins were grouped into families according to PlantsT (http://plantst.sdsc.edu/). Proteins listed in the Aramemnon database (http://aramemnon.botanik.uni-koeln.de/) as isospecific homologs of the phosphoprotein with at least 30% similarity were called protein family. Under the heading Conservation within Protein Family, the number of family members that share the phosphorylation site is listed. Under the heading Conservation among Putative Orthologs, (+) denotes one BLAST hit in the EST database with conserved phosphorylation site; + denotes two or more hits; n.f., no ambiguous ortholog was found in the EST databases.