Table 1.
CART peptide-like immunoreactive (CART-LI) perikarya and nerve fibers in porcine stomach, duodenum, and descending colon under physiological conditions (C group) and after T-2 toxin administration (T2 group)
| Stomach | C group | T2 group |
|---|---|---|
| CMLa | 13.36 ± 0.75 | 20.25 ± 1.32 |
| MP | ||
| CBb | 46.24 ± 2.12 | 63.59 ± 1.12 |
| NFc | + | ++ |
| SP | ||
| CBb | 6.39 ± 0.17 | 16.33 ± 1.30 |
| NFc | + | + |
| S/MLa | 0.83 ± 0.25 | 2.92 ± 0.45 |
| Duodenum | ||
| CMLa | 15.99 ± 0.80 | 26.66 ± 1.73 |
| MP | ||
| CBb | 38.10 ± 3.43 | 63.80 ± 0.83 |
| NFc | +++ | +++ |
| OSP | ||
| CBb | 28.70 ± 0.90 | 53.31 ± 1.49 |
| NFc | ++ | ++ |
| ISP | ||
| CBb | 21.96 ± 1.85 | 35.86 ± 1.85 |
| NFc | ++ | +++ |
| S/ML | 3.07 ± 0.14 | 8.3 ± 1.18 |
| Descending colon | ||
| CMLa | 15.33 ± 1.77 | 23.37 ± 1.04 |
| MP | ||
| CBb | 33.43 ± 3.39 | 47.16 ± 1.30 |
| NFc | +++ | +++ |
| OSP | ||
| CBb | 27.50 ± 1.07 | 41.38 ± 1.79 |
| NFc | ++ | +++ |
| ISP | ||
| CBb | 19.07 ± 4.11 | 42.35 ± 6.18 |
| NFc | + | ++ |
| S/MLa | 1.94 ± 0.35 | 3.75 ± 0.33 |
Differences between C group and T2 group in all parts of gastrointestinal tract studied are statistically significant (p ≤ 0.05)
CML circular muscle layer, MP myenteric plexus, SP gastric submucous plexus, OSP intestinal outer submucous plexus, ISP intestinal inner submucous plexus, S/ML submucosal/mucosal layer, CB cell bodies, NF nerve fibers
aAverage number of nerve profiles per area studied (mean ± SEM)
bRelative frequency of particular neuronal subclasses in presented as % (mean ± SEM) of all neurons counted within the ganglia stained for PGP 9.5 (used as pan neuronal marker). At least 500 PGP 9.5-positive cell bodies were evaluated in particular types of enteric plexuses in each animal
cThe density of intraganglionic nerve fibers positive for CART is presented in arbitrary units