Figure 5.
ttl Overexpression Leads to Growth Inhibition.
(A) RNA gel blot analysis of ttl overexpression in eight independent transgenic lines with the 32P-labeled full-length ttl cDNA probe.
(B) Phenotypic comparison of two ttl-overexpressing transgenic lines with the corresponding wild-type (Col-0) plants. Plants shown here were 5 weeks old grown under long-day growth condition (16 h light/8 h dark).
(C) Quantitative analysis of the hypocotyl growth of the two ttl-overexpressing transgenic lines grown for 5 d in the dark or under a short-day (8-h-light/16-h-dark) growth condition. Hypocotyl growth is expressed as a percentage of the hypocotyl length of the 5-d-old wild-type seedling grown in the dark, and each data point represents the average of ∼50 seedlings.
(D) Quantitative analysis of the height and petiole length of the ttl transgenic plants relative to those of the wild-type plants. The wild-type control and the two ttl-overexpressing transgenic lines were grown in soil for 7 weeks under a long-day (16-h-light/8-h-dark) growth condition. The length of the main inflorescence stem was used as the height of an adult plant, and the average petiole length of the three longest rosette leaves of each analyzed plant was taken as the petiole length. Each data point represents the average of 14 plants, and the error bar denotes standard error.