Table 2.
Complementation Analysis of rax1-1 with cad2-1 and 35S-GSH1
| Genotype (Col-0) | Glutathione (nmols g fwt−1) | n |
|---|---|---|
| cad2-1:rax1-1:APX2LUC | 145 ± 6 (118) | 10 |
| rax1-1:APX2LUC | 123 ± 14 (100) | 7 |
| cad2-1 | 103 ± 10 (84) | 3 |
| APX2LUC | 246 ± 44 (200) | 6 |
| 35S:GSH1:APX2LUC | 378 ± 19 (307) | 3 |
| 35S:GSH1:rax1-1:APX2LUC | 247 ± 9 (201) | 6 |
Total glutathione content in leaves from F1 progeny arising from a cross between cad2-1 and rax1-1 parents compared with the wild type (Col-0/APX2LUC) and the rax1-1 and cad2-1 parents. Glutathione was determined as in Table 1. The numbers in parentheses are the values relative to rax1-1/APX2LUC. The presence of both the rax1-1 and cad2-1 mutation in each selected hybrid was confirmed as correct by sequencing of the PCR-amplified GSH1 gene over both mutations (see Figure 1 and Methods). Similarly, the presence of the 35S-GSH1 transgene (Xiang et al., 2001) was confirmed by PCR between the Cauliflower mosaic virus 35S promoter and the GSH1 coding sequence.