Table 3.
Transcript Levels of Genes Whose Expression, When Compared with Nonstressed Wild-Type Plants, Was Affected in Nonstressed rax1-1 Alone or in Both rax1-1 and cad2-1
| Affymetrix Probe Set | Locus Name | Signal in rax1-1/Signal in Wild Type | t Test | Signal in cad2-1/Signal in Wild Type | t Test |
|---|---|---|---|---|---|
| Higher Transcript Levels in rax1-1 | |||||
| 16031_at | At5g01600 | 3.44 | 0.113 | 0.95 | 0.375 |
| 18933_at | At2g40300 | 2.15 (4.6) | 0.097 | 1.08 (1.1) | 0.153 |
| 15115_f_at | At2g05380 | 2.65 | 0.010 | 2.52 | 0.000 |
| 19577_at | At1g65400 | 2.36 | 0.001 | 3.15 | 0.024 |
| 19267_s_at | At4g02330 | 2.33 | 0.010 | 3.25 | 0.059 |
| 13255_i_at | At4g39640 | 2.16 | 0.045 | 3.17 | 0.023 |
| Lower Transcript Levels in rax1-1 | |||||
| 12521_at | At3g51860 | 0.28 | 0.036 | 0.74 | 0.189 |
| 16038_s_at | At5g66400 | 0.28 (0.45) | 0.171 | 1.42 (1.85) | 0.349 |
| 19646_at | At2g46680 | 0.36 | 0.104 | 1.41 | 0.208 |
| 12212_at | At3g52070 | 0.46 | 0.133 | n.d. | – |
| 15750_at | At2g03980 | 0.46 | 0.016 | 0.52 | 0.017 |
| 19213_at | At2g29580 | 0.48 | 0.010 | 1.08 | 0.278 |
| 19901_at | At2g32880 | 0.48 | 0.039 | 1.05 | 0.373 |
| 13706_at | At2g18700 | 0.48 | 0.091 | 0.51 | 0.099 |
| 18969_g_at | At5g57550 | 0.49 | 0.046 | 0.55 | 0.076 |
| 13146_s_at | At4g35750 | 0.50 | 0.070 | 0.58 | 0.086 |
| 12622_at | At1g23100 | 0.50 | 0.114 | n.d. | – |
| 17840_at | At2g43570 | 0.35 (0.82) | 0.106 | 0.38 (0.18) | 0.115 |
| 19675_at | At4g35580 | 0.40 | 0.198 | 0.39 | 0.195 |
| 13212_s_at | At3g57260 | 0.43 (0.18) | 0.093 | 0.44 (0.08) | 0.094 |
| 17008_at | At2g24850 | 0.44 | 0.028 | 0.21 | 0.017 |
| 14635_s_at | At2g14610 | 0.44 | 0.088 | 0.45 | 0.093 |
| 15141_s_at | At5g24770 | 0.45 | 0.076 | 0.29 | 0.052 |
| 15125_f_at | At5g24780 | 0.47 | 0.058 | 0.20 | 0.030 |
| 18224_s_at | At4g21830 | 0.50 (0.8) | 0.141 | n.d. (0.3) | – |
| 12244_s_at | At2g48110 | 0.50 | 0.177 | 0.48 | 0.167 |
The data were obtained from microarray experiments using a total of three independent preparations of RNA per line prepared from plants grown in controlled environment conditions (see Methods). Locus identifiers in bold indicate genes with altered expression only in rax1-1. The numbers in parentheses are the same comparisons conducted using a qRT-PCR procedure for that gene with two replicate RNA preparations independent of those used for the microarray experiments. n.d., not detected; –, not determined.