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. 2016 Dec 15;8(12):5169–5186.

Figure 6.

Figure 6

Effects of quercetin and PPARγ knockdown on cardiomyocyte oxidative damage after H/R in vitro. (A) Western blot analysis of the expression of iNOS in H9C2. Quantification of the densitometry of western blot bands is shown in (B). Data shown represent mean ± SD. (C) Representative images of DHE staining of H9C2 cells (magnification ×400). (i) H9C2 pretreated with DMSO and cultured in normoxic condition (Sham). (ii) H9C2 transfected NC-siRNA, pretreated with DMSO and suffered from H/R (H/R+NC-siRNA). (iii) H9C2 transfected NC-siRNA, pretreated with quercetin and suffered H/R (H/R+quercetin+NC-siRNA). (iv) H9C2 transfected PPARγ-siRNA, pretreated with quercetin and suffered H/R (H/R+quercetin+PPARγ-siRNA). (v) H9C2 transfected PPARγ-siRNA, pretreated with DMSO and suffered H/R (H/R+PPARγ-siRNA). (D) Quantification of DHE intensities in the indicated groups (five fields for each specimen). The values were presented as mean ± SD. *P<0.05, **P<0.01 vs Sham group; #P<0.05, ##P<0.01 vs IRI group. All experiments were repeated three times.