Figure 1.

Isolation of ACE1 by Complementation.

Schematic representation of the ACE1 locus in Guy11, cosmids, and subclones tested for complementation to avirulence. The genomic locus is represented as a solid line, and the positions of RAPD marker OPE-Y13 and relevant restriction sites are indicated as follows: E, EcoRI; X, XbaI; B, BamHI; N, NotI; Bg, BglII. The ACE1 ORF is indicated as an arrow, and interruptions indicate positions of the three introns. Above the genomic locus map, the relative positions of the noncomplementing cosmid D22F11 and the complementing cosmid D31C12 are shown. Below the genomic locus map, the relative positions of D31C12 subclones used for complementation are shown. Fractions given to the right of each construct indicate the number of avirulent transformants identified over the number of transformants tested. Numbers without asterisks represent results obtained with virulent parent 2/0/3, numbers with asterisks represent results obtained with virulent field isolate PH14. Cosmid D31C12 and plasmid pEco20 complement both virulent strains to avirulence, demonstrating that they carry ACE1.