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. 2004 Sep;16(9):2499–2513. doi: 10.1105/tpc.104.022715

Figure 4.

Figure 4.

Expression Pattern of ACE1 in Avirulent Isolate Guy11.

(A) PCR and RT-PCR amplification of the constitutively expressed ACT1 gene as control of RNA integrity. The region spanning the first three introns of ACT1 was amplified. Lane 1, ACT1 PCR product obtained from genomic DNA (size, 900 bp). Lane 2, ACT1 RT-PCR product obtained from RNA isolated from barley leaves infected with Guy11 17 h after infection (size, 350 bp). Lane 3, ACT1 RT-PCR product obtained from RNA isolated from Guy11 mycelial culture. The amplification products were separated by agarose gel electrophoresis and stained with ethidium bromide.

(B) PCR and RT-PCR amplification of ACE1 from avirulent parent Guy11. The region spanning the first intron of ACE1 was amplified. Lane 1, ACE1 PCR product obtained from genomic DNA (size, 453 bp). Lane 2, ACE1 RT-PCR product obtained from RNA isolated from barley leaves infected with Guy11 17 h after inoculation (size, 365 bp). Lane 3, No ACE1 RT-PCR product was obtained with RNA isolated from Guy11 mycelial culture, suggesting that ACE1 is not expressed in mycelium.