. 2004 Sep;16(9):2499–2513. doi: 10.1105/tpc.104.022715

Table 4.

Primers Used in This Study

Primer	Sequence (5′/3′)	Purpose
H-OL1	`TTGCCTCCATTGTGCTGAAG`	RT-PCR (amplification of ACE1 intron 1)
H-OL2	`GCCTTCTGTGTGCCCAAT`	RT-PCR (amplification of ACE1 intron 1)
H-OL3	`CCGCCGTCGTCACTCCCACC`	RT-PCR (amplification of ACE1 intron 2)
H-OL4	`TGACAGAGGACAGGAAGACG`	RT-PCR (amplification of ACE1 intron 2)
H-OL5	`CTCTTCTCGGTCGGCAACAC`	RT-PCR (amplification of ACE1 intron 3)
H-OL6	`CGACGGCGACCAGTGAATCC`	RT-PCR (amplification of ACE1 intron 3)
H-OL7	`ACATTGCTGGTTCCGTGGTGTTTATGA`	5′ RACE of ACE1
H-OL8	`CATCACGGGCGAGGGCACCAAT`	3′ RACE of ACE1
H-OL9	`TTGCGATTAGCGTCCATTGT`	RT-PCR (amplification of ACT1 introns 1 to 3)
H-OL10	`TTCATCAGGTAGTCGGTCAA`	RT-PCR (amplification of ACT1 introns 1 to 3)
H-OL11	`CGGAAATCCGCATCTCTAGCTTGGCGC`	Construction of pProACE1:GFP
H-OL12	`GTCACCCATGGTTGGTGATTTATTTGTTT`	Construction of pProACE1:GFP
H-OL13	`GGCCGGC`	Construction of pProACE1:GFP
H-OL14	`GCCACTCGGAATAAACAGAGGGTGGTC`	Construction of pProACE1:GFP
H-OL15	`GCTGAAGGGCGGCCGCTGATTCATGAACTTTGGAATACTGTAACTAG`	Construction of pACE1:GFP
H-OL16	`CAGTATTCCAAAGTTCATGACCATGGTC`	Construction of pACE1:GFP
H-OL17	`CCGTCCTTCAGC`	Construction of pACE1:GFP
H-OL18	`GCTGAAGGACGGGACCATGGTCATGAA`	Construction of pACE1:GFP
H-OL19	`CTTTGGAATACTG`	Construction of pace1^C183A:GFP
H-OL20	`GCTGAAGGGCGGCCGCTGATTCATGAACTTTGGAATACTGTAACTAG`	Construction of pace1^C183A:GFP
	`GCCACTCGGAATAAACAGAGGGTGGTC`
	`CGGTCACGGTCGATACCGCAGCTAGCTCCAGCTTGGTGGCCG`
	`CGGCCACCAAGCTGGAGCTAGCTGCGGTATCGACCGTGACCG`