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. 2016 Dec 15;8(12):5338–5353.

Figure 3.

Figure 3

Eotaxin-2 Prolongs TLR4 mRNA stability and promotes TLR4 expression. A: Following treatment of HCAECs with eotaxin-2 for 30-240 minutes, the phosphorylation of p38 MAPK, JNK/SAPK and ERK1/2 were analyzed by Western blot. The total p38 MAPK, JNK/SAPK, ERK1/2, and β-actin protein levels were used as loading controls. B: HCAECs were pretreated with SB203580, SP600125, or PD98059 for 1 hour prior to treatment with eotaxin-2 for 8 hours. The expression levels of TLR4 were analyzed by quantitative real time PCR. C: HCAECs were pretreated with SB203580, SP600125, or PD98059 for 1 hour prior to treatment with eotaxin-2 for 4 hours. The half-life of TLR4 mRNA were analyzed by actinomycin D chase experiment. D: HCAECs were pretreated with SB203580, SP600125, or PD98059 for 1 hour prior to treatment with eotaxin-2 for 18 hours. The total TLR4 expression was analysis using western blotting. All bars represent the results of three independent experiments. The data are presented as the mean ± SEM and statistical evaluation was performed using one-way ANOVA followed by a Dunnett’s test. *P<0.05 was considered significant.