Figure 3.

Construction and screening of cDNA library. A. Total RNAs from tissues of hypertrophic zone (HZ) and from hypertrophic MCT cells were reversely transcribed and PCR amplified using specific random primer (CDSIII/6). Most of the cDNA fragments (PCR products) are around 500-bp. B. The library cDNA and the PGBKT7-Runx2 bait plasmid were co-transformed and plate on selective media (SD/-Leu-His-Trp-Ade/ABA/X-αgal) for selection of target colonies. C. The target colonies showing potential interaction were further re-streaked on the same selective medium to confirm the interaction and for further analysis.