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. 2016 Dec 15;8(12):5569–5579.

Figure 1.

Figure 1

Advanced glycosylation end product (AGE) inhibits capacities of epidermal stem cells (ESCs). Rat ESCs were isolated and cultured with AGE-modified bovine serum albumin (AGE-BSA) or BSA (as a control) treatment. Cells were detected by qRT-PCR, flow cytometry and reactive oxygen species (ROS) assay after 48 h of treatment. A. qRT-PCR shows that mRNA levels of ESC markers integrin β1 (Itgb1) and keratin 19 (Krt19) are suppressed by AGE-BSA treatment. B. Flow cytometry shows that the percent of apoptotic cells is increased by AGE-BSA treatment. C. MTT assay shows that the viability of ESCs is inhibited by AGE-BSA treatment when detected at 1, 2 and 3 d post treatment. D. ROS level is elevated by AGE-BSA treatment. **P < 0.01 and ***P < 0.001 compared to the BSA group.