Figure 2.

Advanced glycosylation end product (AGE) elevates expression of AGE-specific receptor (AGER) and forkhead box O1 (FOXO1) in epidermal stem cells (ESCs). Rat ESCs were isolated and cultured with AGE-modified bovine serum albumin (AGE-BSA) or BSA (as a control) treatment. Cells were detected by qRT-PCR and Western blot after 48 h of treatment. A. qRT-PCR shows that mRNA levels of Ager and Foxo1 are elevated by AGE-BSA treatment. *P < 0.05 and ***P < 0.001 compared to the BSA group. B. Western blot shows the expression of AGER and FOXO1 proteins are increased by AGE-BSA treatment. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an internal reference.