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. 2016 Dec 15;8(12):5569–5579.

Figure 3.

Figure 3

Knockdown of advanced glycosylation end product-specific receptor (AGER) improves capacities of epidermal stem cells (ESCs). Rat ESCs were isolated and cultured with AGE-modified bovine serum albumin (AGE-BSA) for 48 h, after which they were transfected with the specific small interfering RNA for Ager (si-AGER) or the control (si-control). Detection of AGER levels by qRT-PCR (A) and Western blot (B) at 48 h post transfection suggests the successful knockdown of AGER. Meanwhile, forkhead box O1 (FOXO1) is also suppressed. (C) qRT-PCR shows that mRNA levels of ESC markers integrin β1 (Itgb1) and keratin 19 (Krt19) are suppressed by AGER knockdown at 48 h post transfection. (D) MTT assay shows that cell viability is elevated by AGER knockdown when detected at 1, 2 and 3 d post transfection. (E) Flow cytometry indicates that the percent of apoptotic cells is reduced by AGER knockdown at 48 h post transfection. (F) ROS level is suppressed by AGER knockdown at 48 h post transfection. *P < 0.05 and **P < 0.01 compared to the si-control group.