Figure 5.

Forkhead box O1 (FOXO1) competes with lymphoid enhancer binding factor 1 (LEF1) to interact with β-catenin protein. Rat epidermal stem cells were isolated and cultured with AGE-modified bovine serum albumin (AGE-BSA) or BSA (as a control) treatment for 48 h, after which they were transfected with the specific small interfering RNA for Ager (si-AGER) or the control (si-control). Immunoprecipitation was performed at 48 h post transfection with the antibodies against β-catenin. Immunoprecipitation products were detected by Western blot with primary antibodies against FOXO1 and LEF1.