Table 1.
Primers used in qRT-PCR
| Primer | Targeted gene (GenBank Accession) | Sequence (5’ to 3’) | Produce size (bp) |
|---|---|---|---|
| Itgb1-Fw | Itgb1 (NM_017022) | GGGACACGGGTGAAAATCCT | 152 |
| Itgb1-Rv | AGAGCCCCAAAGCTACCCTA | ||
| Krt19-Fw | Krt19 (BC126075) | CATGGCAGAGAAGAACCGGA | 125 |
| Krt19-Rv | GGAGTTCCGTGACCTCAGTC | ||
| Ager-Fw | Ager (NM_053336) | GTCTCCTTCAGCTTCCGACC | 117 |
| Ager-Rv | AGCATGGATCATGTGGGCTC | ||
| Foxo1-Fw | Foxo1 (NM_001191846) | CAGCCAGGCACCTCATAACA | 143 |
| Foxo1-Rv | TCAAGCGGTTCATGGCAGAT | ||
| Wnt1-Fw | Wnt1 (NM_001105714) | CGTTGCTGTCCCTGTGGTAT | 105 |
| Wnt1-Rv | CAGGTGTGGTGGTTAGGGAC | ||
| Wnt3a-Fw | Wnt3a (NM_001107005) | GACCTTGAGGCCACGTTACA | 90 |
| Wnt3a-Rv | TTGGGCTCGCAGAAGTTAGG | ||
| Ctnnb1-Fw | Ctnnb1 (NM_053357) | ACTCCAGGAATGAAGGCGTG | 109 |
| Ctnnb1-Rv | GAACTGGTCAGCTCAACCGA | ||
| Myc-Fw | Myc (NM_012603) | TGAAAAGAGCTCCTCGCGTT | 139 |
| Myc-Rv | AAATAGGGCTGCACCGAGTC | ||
| Ccnd1-Fw | Ccnd1 (NM_171992) | TCAAGTGTGACCCGGACTG | 138 |
| Ccnd1-Rv | GACCAGCTTCTTCCTCCACTT | ||
| Mmp7-Fw | Mmp7 (NM_012864) | CAAAGGACGACATTGCAGGC | 94 |
| Mmp7-Rv | GAAGGGCGTTTGCTCATTCC | ||
| Gapdh-Fw | Gapdh (NM_017008) | TCCTGCACCACCAACTGCTTAG | 102 |
| Gapdh-Rv | AGTGGCAGTGATGGCATGGACT |