Figure 2.

Calmodulin cDNA enhances calmodulin expression and CaM shRNA knocks down calmodulin expression in both HEK293 and neuron cells. A. qRT-PCR and Western blots of calmodulin from HEK293 cells in control and with transfection of calmodulin cDNA and shRNA. B. qRT-PCR and Western blots of calmodulin from neuron cells in control and with transfection of calmodulin cDNA and shRNA. C. Relative level of mRNA and protein after transfection with CaM cDNA and shRNA in HEK293 cells. D. Relative level of mRNA and protein after transfection with CaM cDNA and shRNA in neuron cells. β-actin and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a loading control for RT-PCR and western-blot, respectively.