Figure 1.

Allergic rhinitis model. On days 1, 7, 14, and 21, mice received an intraperitoneal (ip) injection of 40 µg/kg of ovalbumin (OVA) and 1 mg of alum in 200 µl of PBS. One week after the last OVA sensitization (day 21), a series of 14 daily intranasal (in) OVA (20 µl of 25 mg/ml) challenges were administered. The mice were killed 24 h after the last nasal challenge for further analyses.