Figure 4.

Pyroptosis-associated protein inhibitors can alleviate acute liver injury induced by sepsis. Inhibitors of capase-1 (AC-YVAD-CMK), NLRP3 (Glyburide) alone or in combination were administrated to mouse via intraperitoneal injection before CLP: (A) The 10 days survival rate in each group; (B) The HE staining of liver tissues (100×) of each group; (C, D) The concentrations of IL-1β and IL-18 in the serum (C) and ALT & AST (D) were examined by ELISA; (E) The expressions of pyroptosis-related proteins were examined by Western blot; (F) Hepatic cell pyroptosis was examined by flow cytometry, left: two-dimensional graphs, right: histogram. *P < 0.05, **P < 0.01, vs. sham operation group; %P < 0.05, %%P < 0.01, vs. CLP group.