Figure 3.

IL-4 induces chromatin remolding at miR-98 promoter locus to increase miR-98 expression in B cells. Peripheral B cells were collected from 6 healthy subjects and stimulated with IL-4 in the culture at graded doses (as denoted on the X axis of each panel) for 48 h. The B cells were analyzed by ChIP (A-D). The bars indicate the levels of p300 (A), H3K4 (B) and RNA polymerase II (Pol II; C) at the miR-98 promoter locus. (D) The bars indicate the miR-98 levels in the B cells. Data of bars are presented as mean ± SD. *, P<0.01, compared with the dose “0” group. Samples from individual subjects were analyzed separately (The data are summarized from 6 independent experiments). #, the presence of garcinol (an inhibitor of p300; 10 μM).