Figure 9.

Phenotypes of Ectopic HAN Expression.

(A) Lobed cauline leaf from a 35S:HAN plant. Inset shows a wild-type cauline leaf.

(B) Wild-type inflorescence.

(C) 35S:HAN inflorescence.

(D) Wild-type (left) and 35S:HAN flowers.

(E) Wild-type (right) and 35S:HAN siliques.

(F) to (H) 35S:HAN-GR transformants.

(F) DEX-treated inflorescence has early opening flowers and a short stem.

(G) Seedlings mock-treated from at 11 d of age, shown at 29 d old.

(H) Seedlings treated with DEX beginning at 11 d of age, shown at 29 d old. Arrow points to lobed leaf margins.

(I) Homozygous 35S:HAN-GR seedling germinated on a DEX plate at 9 d.

(J) SAM of 10-d-old wild-type seedling. M, SAM.

(K) and (L) SAMs of 10-d-old 35S:HAN-GR seedlings germinated on DEX plates. Arrows point to the SAMs.

(M) WUS expression in a 10-d-old 35S:HAN-GR seedling germinated on non-DEX plates.

(N) Absence of WUS expression in a 10-d-old 35S:HAN-GR seedling germinated on DEX plates.

(O) Non-DEX-induced control stomata with two guard cells.

(P) and (Q) Guard cells in 10-d-old and 6-d-old DEX-treated 35S:HAN-GR seedlings, respectively. Arrows point to stomata with five or seven guard cells.

(R) Mock-treated 35S:HAN-GR root tip. Arrow points to the root cap.

(S) DEX-induced 35S:HAN-GR root tip.

Bars in (A) to (I) = 1 mm; bars in (J) to (S) = 50 μm (except for [Q], in which the bar = 10 μm).