Fig. 4.

EPR spectra of the Dre2 variants mutated in the CXnCX₂CXC domain (A site mutated, B site unmutated) at 6 K, 5 mW (A) and at 40 K, 1 mW (B). C252A, C263A, C266A and C268A Dre2 mutants were overexpressed and purified from Escherichia coli. The samples were reduced with 10 mM sodium dithionite. EPR spectra from these Dre2 mutants are shown in red, whereas EPR spectra from WT are shown in black for comparison. EPR conditions are the same as described in Fig. 3.