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. 2016 Sep 26;161(1):67–78. doi: 10.1093/jb/mvw054

Fig. 9.

Fig. 9

Yeast shuffle strains show that both clusters are essential for viability. (A) Yeast Dre2. Plasmids carrying various Dre2 alleles including WT and Cys to Ala mutants along with the pRS415 plasmid were transformed into the Dre2 shuffle strain and selected on CSM-Leu-Ura medium. The transformants were then passaged on FOA medium to counterselect against the URA3-containing pRS416-Dre2 plasmid. (B) hDre2. YCPlac22 plasmids carrying various human Dre2 (anamorsin) alleles including WT and Cys to Ala mutants were transformed into the Dre2 shuffle strain and selected on CSM-Trp-Ura medium. The transformants were then passaged on FOA medium to counterselect against the URA3-containing pRS416-Dre2 shuffle plasmid.