Figure 5.

Posttranscriptional Gene Silencing Is Specific to AGP18.

RNA extracted from developing gynoecia of selected AGP18-RNAi T2 lines (T2-12, T2-57, and T2-58) was used for cDNA synthesis. PCR amplification was performed with primers specific to AGP17, AGP18, or AGP19 (Schultz et al., 2002) using as a template samples corresponding to the same cDNA synthesis. Agarose gels were blotted on nitrocellulose membranes and probed with a corresponding AGP probe. Wild-type cDNA and amplification of ACT11 were used as positive controls.