Figure 6.

Gadolinium and PPADS Suppress ATP-Induced Changes in Gene Expression.

Blots were stripped and rehybridized with different probes as indicated. Quantitations of mRNA levels were performed by densitometry using a PhosphorImager and normalized against the ribosomal 25S rRNA. Transcript level values are shown relative to the control.

(A) Gadolinium (Gd³⁺; 10 mM) and 300 μM PPADS pretreatment reduce the ATP-mediated increase in the level of mRNAs encoding various MAPKs in Arabidopsis suspension culture cells treated with 500 μM ATP for 30 min.

(B) Gadolinium (Gd³⁺; 10 mM) and 300 μM PPADS pretreatment reduces the ATP-mediated increase of ERF4 mRNA levels in Arabidopsis suspension culture cells treated with 500 μM ATP for 15 min.