Figure 5.

Expression Analysis of the CDKB1;1 Gene.

(A) Transcript levels of CDKB1;1 during leaf development. cDNA prepared from the first leaf pair harvested at the indicated time points was subjected to semiquantitative RT-PCR analysis with gene-specific primers. The actin 2 (ACT2) gene was used as loading control. DAS, days after sowing.

(B) RNA gel blot analysis of CDKB1;1. RNA was extracted from 12-d-old control (Col-0) and E2Fa-DPa seedlings. Equal loading of the gel was confirmed by methylene blue staining of the membrane (bottom panel).