Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
In Vitro Assay of Elongase Activity.
Microsomes from developing embryos were incubated with 18:4-CoA in the absence (A) and presence (B) of malonyl-CoA, NADH, and NADPH. After purification of the acyl-CoAs, they were converted to their etheno-derivatives and analyzed by HPLC. In the assay in (B), ∼50% of 18:4-CoA is elongated to 20:4-CoA.
