Figure 3. Expression analysis of downregulated genes as a proof of functionality of the RNAi construct.
RT-PCR of COMT (a) and C4H (b) gene along with actin gene as loading control. Northern blot with [α32P] labeled cDNA probe of COMT (c) and C4H gene (d). For hp-northerns, the probes were hpRNAi amplicon specific sequences of COMT (e) and C4H (f) genes respectively. The figures show intensities of bands normalized with respect to 28S rRNA.