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. 2017 Jan 4;7:39984. doi: 10.1038/srep39984

Figure 3. Expression analysis of downregulated genes as a proof of functionality of the RNAi construct.

Figure 3

RT-PCR of COMT (a) and C4H (b) gene along with actin gene as loading control. Northern blot with [α32P] labeled cDNA probe of COMT (c) and C4H gene (d). For hp-northerns, the probes were hpRNAi amplicon specific sequences of COMT (e) and C4H (f) genes respectively. The figures show intensities of bands normalized with respect to 28S rRNA.