Table 1.
Specific Activities of DHS and HSS Encoded by cDNA Sequences Identified in Various Angiosperm Species
| cDNA | Size of ORF (bp) | DHS Activity (pkat/mg) | HSS Activity (pkat/mg) |
|---|---|---|---|
| HSS Heliotropium indicum | 1095 | 0.000 | 0.666 |
| DHS H. indicum | 1116 | 0.155 | 0.238 |
| HSS Cynoglossum officinale | 1110 | 0.000 | 0.902 |
| DHS C. officinale | 1119 | 0.036 | 0.113 |
| HSS Symphytum officinale | 1128 | 0.000 | 0.267 |
| DHS S. officinale | 1119 | 0.165 | 0.479 |
| HSS Eupatorium cannabinum | 1128 | 0.000 | 0.597 |
| DHS E. cannabinum | 1125 | 0.022 | 0.139 |
| HSS Senecio jacobaea | 1119 | 0.000 | 0.328 |
| HSS1 Senecio vernalis | 1113a | 0.000 | 1.124 |
| HSS2 S. vernalis | 1113 | 0.000 | 1.183 |
| DHS S. vernalis | 1116b | 0.147 | 0.280 |
| HSS Senecio vulgaris | 1113c | 0.000 | 0.293 |
| HSS Petasites hybridus | 1110 | 0.000 | 0.652 |
| DHS P. hybridus | 1098 | 0.018 | 0.048 |
| DHS Ipomoea hederifolia | 1149 | 0.147 | 0.631 |
| DHS Nicotianum tabacum | 1140d | 0.089 | 0.406 |
| DHS Crotolaria retusa | 1116 | 0.016 | 0.030 |
| HSS Phalaenopsis sp | 1116 | 0.000 | 0.504 |
The size of the ORF expressed heterologously in E. coli is given for each sequence, as are the specific activities according to the DHS and HSS assays of desalted crude extracts of E. coli expressing the respective cDNA. For SDS-PAGE analysis of the assayed E. coli cultures, see Supplemental Figure 1 online.