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. 2017 Jan 4;7:39768. doi: 10.1038/srep39768

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(a) Selection of induction medium. Enzyme expression was higher in LB. However, cell mass was higher in Terrific broth. Itaconate/DCW/Hour (); DCW (). (b) Determination of the optimal citrate concentration; 500 mM citrate produced the highest amount of itaconate. The reaction was conducted at pH 7 and 35 °C for 24 h. Itaconate concentration (bar); Conversion (⚫).

Inline graphic — (a) Selection of induction medium. Enzyme expression was higher in LB. However, cell mass was higher in Terrific broth. Itaconate/DCW/Hour (); DCW (). (b) Determination of the optimal citrate concentration; 500 mM citrate produced the highest amount of itaconate. The reaction was conducted at pH 7 and 35 °C for 24 h. Itaconate concentration (bar); Conversion (⚫).