Table 2. Number of predicted RNA features for different read cut off and length filters.
| Min counts | Min length (nt) | Max length (nt) | No of features | No of DE featuresa |
|---|---|---|---|---|
| 1000 | 15 | 30 | 414 | 3 |
| 1000 | 15 | 25 | 385 | 3 |
| 1000b | 16 | 25 | 325 | 3 |
| 500 | 15 | 30 | 669 | 7 |
| 500 | 15 | 25 | 623 | 7 |
| 500 | 16 | 25 | 536 | 6 |
| 200 | 15 | 30 | 1211 | 12 |
| 200 | 15 | 25 | 1149 | 12 |
| 200 | 16 | 25 | 1016 | 12 |
| 100 | 15 | 30 | 1876 | 24 |
| 100 | 15 | 25 | 1790 | 23 |
| 100 | 16 | 25 | 1586 | 22 |
| 50c | 15 | 30 | 2820 | 46 |
| 50 | 15 | 25 | 2699 | 44 |
| 50 | 16 | 25 | 2425 | 42 |
aNumber of differential expression (DE) of small RNAs isolated from MalaEx harvested from 5 pairwise M. sympodialis cultures at pH 5.5 and pH 6.1, respectively (see Table 1). bThe most stringent specifications. cDataset selected for DE analysis and mapping to the human genome.