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. 2017 Jan 4;7:39222. doi: 10.1038/srep39222

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Copyright © 2017, The Author(s)

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(a) PDCD5 interacts with PPEF-1, PPP1CA, PPP6C, and PPM1K. Myc-tagged PDCD5 and Flag-tagged phosphatases were co-transfected into HCT116 (p53^+/+) cells. Whole-cell lysates were immunoprecipitated and immunoblotted with the indicated antibodies. (b) PPEF-1 selectively dephosphorylates PDCD5 at Ser-119. Cell lysates were immunoblotted with the indicated antibodies. (c) The IQ motif domain of PPEF-1 interacts with PDCD5. Schematic diagrams of Myc-tagged PPEF-1 for GST pull-down analysis. Bound proteins were eluted and analyzed using autoradiography. (d) The N-terminal domain of PDCD5 (residues 1–30) interacts with PPEF-1. Schematic diagrams of Myc-tagged PDCD5 for co-immunoprecipitation and mapping analysis. HCT116 (p53^+/+) cells were cotransfected with the indicated Myc-PDCD5 and Flag-PPEF-1 plasmids. Cell lysates were immunoprecipitated with Flag antibodies, and subsequently immunoblotted with the indicated antibodies. Full-length blots are presented in Supplementary Figure 1a-d.