(A) The XICs of the NAT and SIL species of H5N4F2X1, an N-glycan identified for the first time across any species, co-elute (16.1 – 16.5 min) and are observed in high abundance.
(B) An annotated, example MS/MS spectrum from NAT tagged H5N4F2X1, with the regions from 1000–2000 m/z and 500–1000 m/z highlighted, is given. The assignments across all H5N4F2X1 MS/MS spectra observed are provided in Table S1.