Table 2.
List of monolignol proteins identified by a motif containing deamidated peptide in PNGase F/Glycosidase A treated samples are shown. Additional information regarding the peptides presence in control samples and site occupancy based on peptide spectral matches (PSMs) yields information regarding confidence in these as possible glycosylation sites.
| Name | In Control | Peptides containing motif | PSM Occupancy | PSMs |
|---|---|---|---|---|
| CAld5H2 | No | KQNNFSEDAETDMVDDMLAFYSEEAR (Glyc A only) | 43% | 3 |
| HCT1 | No | AKEDGNNISYSSYEMLAAHVWR (PNGase F only) | 50% | 2 |
| PAL1 | No | FLNAGIFGNGTETCHTLPHSATR (PNGase F only) | 13% | 1 |
| PAL3 | No | FLNAGIFGNGTETCHTLPHSATR (PNGase F only) | 13% | 1 |
| PO42 | No | DFNNTTVLDIR (GlycA only) | Deamidated only | 13 |
| No | DFNNTTVLDIR (GlycA only) | Deamidated only | 5 | |
| No | DFNNTTVLDIR (GlycA only) | Deamidated only | 3 | |
| No | FATQNETLDNLPPPFANADTILSSLATK | 98% | 53 | |
| C3H | No | LEALRPIREDEVAAMVESIFNDCTNPENNGK | 27% | 3 |
| LIM2 | No | EKGNLSQLEGDIEK | Deamidated only | 1 |
| IRX10-2 | No | SAIQLLSSNWPYWNR | Deamidated only | 6 |
| CAD2 | Yes | VGVGCLVGACHSCESCASDLENYCPK (PNGase F only) | 33% | 2 |
| CCR | Yes | EAIQGCDGVFHTASPVTDDPEEMVEPAVNGTK | 13% | 1 |
| CesA17 | Yes | VSAVLTNAPFMLNLDCDHYVNNSK | 40% | 2 |
| 4CL3 | Yes | NLPLHSYVLENLSK (Glyc A only) | Deamidated only | 1 |
| CCoAOMT1 | Yes | VGGLIGYDNTLWNGSVVAPPDAPMRK | 17% | 18 |
| CCoAOMT2 | Yes | VGGLIGYDNTLWNGSVVAPADAPMRK | 3% (N9) and 35% (N13) | 2 (N9), 22 (N13) |
| PAL4 | Yes | FLNAGIFGNGTESSHTLPR | 29% | 9 |
| PAL5 | Yes | FLNAGIFGNGTESSHTLPR | 29% | 9 |