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. 2017 Jan 3;13:3. doi: 10.1186/s13007-016-0151-5

Table 1.

List of primer sequences used in this study

Primer Sequence (5′–3′)
VIGS construct
CS_lic_F CGACGACAAGACCCTGAGAAGGGAAACTGCCATTTGA
CS_An2_R CTTTGTCTAGTG CCTTGCCCAGCTTTGTAATCTT
GFP_lic_F CGACGACAAGACCCTCACGGCAGACAAACAAAAGA
GFP_An2_R CTTTGTCTAGTG AAAGGGCAGATTGTGTGGAC
An2_F CACTAGACAAAGACGAACGCGAC
An2_lic_R GAGGAGAAGAGCCCTCAGAAAAGTCATCCCAACCATCAC
PDS_lic_F CGACGACAAGACCCTCTTGCAAAGATCCCCTGTAG
PDS_An2_R CTTTGTCTAGTGCACTTGTTTCTGCCAACTTC
Sequencing
TRV2_seq_F CTGTTTGAGGGAAAAGTAG
TRV2_seq_R CAAAAGACTTACCGATCAATC
qRT-PCR
CS_F TTCCCATATAGCCCACTTGC 
CS_R ACTACAAGCAAATTACCACCTTC
PDS_F AGCAAAGCAAAAATATTGAAGTA
PDS_R GCTTTCCTGATAAGACAGC
An2_F GGAGAAGGAAAGTGGCATCTTGT
An2_R CACCTCTCTTTATATGCGGCCTT
CaActin_F ATGGCAGATGAAGATATTCAAC
CaActin_R ACTAGGAAAAACAGCCCTTGGT