Fig. 4.

A and B. 5-HT-induced GLUT-4 translocation is reduced by MDC. L6 cells were incubated without (a) or with (b) 100 nM insulin for 1 h. Incubations were performed with 25 µM MDC, 1 µM pargyline (P) or without either of these substances (C), in the absence or presence of 10 µM 5-HT. Cell lysates were used for membrane protein isolation followed by immunoblotting (IB) with an antibody against GLUT4 (N = 3). Detection of pan-cadherin (p-Cad) was used as a loading control. Significant differences are indicated as ^**P < 0.01. c 5-HT-induced glucose uptake is reduced by MDC. L6 cells were incubated without or with 100 nM insulin for 1 h. Incubations were performed with 25 µM MDC, 1 µM pargyline (P) or without either of these substances (C), both in the absence or presence of 10 µM 5-HT. Significant differences are indicated as ^*P < 0.05, ^***P < 0.001 (N = 3 in triplicate). d 5-HT-induced glycogen content is reduced by MDC. L6 cells were incubated without or with 100 nM insulin for 3 h. Incubations were performed with 25 µM MDC, 1 µM pargyline (P) or without either of these substances (C), in the absence or presence of 10 µM 5-HT. Significant differences are indicated as ^*P < 0.05, ^***P < 0.001 (N = 3 in triplicate)