Fig. 5.

EBF1 and ZNF423 are potential upstream regulators of M3 gene expression. EBF1 and ZNF423 are potential upstream regulators of M3 gene expression. a Enrichment test for TF-binding motifs in the 10kb up- and down-stream of transcription start sites identify two TFs, ZNF423 (blue) and EBF1 (orange), that are members of the M3 module. b EBF1 and ZNF423 are predicted to target many of the same M3 genes. Circles in the Venn diagram represent the number of genes targeted by TFs and their overlap – EBF1 (orange), ZNF423 (blue), and when both have motifs directly adjacent to each other (anchored analysis, yellow). c ZNF423 and EBF1 expression is highly correlated with M3 eigengene expression. Points represent individual samples. d TF-binding motif density is greatest immediately upstream of M3 transcription start sites. Coloured lines represent the density TF motifs for EBF1 (orange), ZNF423 (blue) and the combination of both (green)