Figure 1.

Endotoxin and mechanical stress induces decreases in DNA methylation in the nicotinamide phosphoribosyltransferase (NAMPT) promoter. Bisulfite sequencing of DNA from human lung endothelial cells (ECs) demonstrated decreases in DNA methylation occurring in both of the CpG islands of the NAMPT promoter. Compared with unstretched or unstimulated cells, 18% cyclic stretch (CS), and endotoxin challenges (100 ng/mL), DNA methylation was significantly decreased. Blue bars depict unmethylated alleles, and red bars depict methylated alleles. Each row is representative of a unique allele, and each column depicts individual CpG sites.